| ID | Gladiator | Real name | Image | | 1 | Apollo | Alex Gray |  | Former Rugby union and NFL player |
| 2 | Bionic | Matty Campbell |  | Bodybuilder, former sprinter and footballer |
| 3 | Giant | Jamie Christian Johal |  | Professional bodybuilder and former firefighter |
| 4 | Legend | Matt Morsia |  | Bodybuilder, powerlifter, and former PE teacher, long and triple jumper |
| 5 | Nitro | Harry Aikines-Aryeetey |  | Team GB Commonwealth, World, and European champion sprinter |
| 6 | Phantom | Toby Olubi |  | Olympics bobsledder and sprinter |
| 7 | Athena | Karenjeet Kaur Bains |  | Commonwealth, All-England and British Powerlifting Champion |
| 8 | Comet | Ella-Mae Rayner |  | Former elite gymnast and cheerleader |
| 9 | Diamond | Livi Sheldon |  | Professional bodybuilder and personal trainer |
| 10 | Dynamite | Emily Steel |  | Elite CrossFit athlete and National Swimming Champion |
| 11 | Electro | Jade Packer |  | Sports model, fitness coach, bodybuilder and hybrid-style athlete |
| 12 | Fire | Montell Douglas |  | Represented Team GB at Olympic sprinting and bobsleigh |